December 9th, 2025

Phosphoramidites & Solid Supports
for Oligonucleotide Synthesis

emp BIOTECH supplies a comprehensive range of raw materials for DNA and RNA synthesis, including high-quality phosphoramidites, CPG supports, and polystyrene solid supports. Our product portfolio is sourced through a controlled network of selected manufacturing partners, including U-Genes Biotechnology, whose materials meet the performance, documentation, and consistency standards required by emp BIOTECH.

All products distributed through emp BIOTECH follow our established quality framework, ensuring reliable integration into automated and manual oligonucleotide synthesis workflows. More

March 7th, 2025

SMIRF™- the fast way to optimize protein purification.
When faced with optimizing purity and yield of your target protein, it can be daunting, time-consuming (and expensive) to test several different columns and resins.
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With SMIRF kits, you get a number of pre-packed FPLC columns, spanning the range of binding modalities and strengths – in one kit. Start right away, whether you are interested in antibody purification, IEX, HIC or IMAC.

February 25th, 2025

SMART Chromatography™ workshop
Alistair Hurst will be leading a workshop on the SMART Chromatography™ platform.
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Arranged in collaboration with the UNSW Recombinant Protein Facility and our distributor in Australia, Metagene, this workshop will present the advantages of the platform in developing more cost efficient and efficient downstream purification processes.

Location of the event
University of New South Wales
Recombinant Protein Facility

Date and time
Tuesday, 25 February 2025
09:30 to 14:00

20th August 2024

EZ Dry Ultra: Setting a New Standard in Solvent Drying and Moisture Control for Oligonucleotide Synthesis Reagents. More

Maintaining a low moisture content (≤ 20 ppm water) in reagents is crucial for the success of oligonucleotide synthesis. For decades, molecular sieves have been the preferred method for achieving and maintaining such low moisture levels in solvents. However, traditional molecular sieves often require significant time and energy to prepare, and they lack objective quality control measures.

emp Biotech’s new EZ Dry Ultra moisture traps revolutionize this process. These ready-to-use molecular sieves are conveniently packaged in sachets, and designed to fit a wide range of solvent volumes. Each sachet features a permeable membrane, allowing moisture to be efficiently adsorbed from the surrounding solvent.

With the opening of emp Biotech’s new manufacturing facility dedicated to EZ Dry Ultra moisture traps, laboratories now have access to a solvent drying product that offers superior quality, consistent production standards, and exceptional convenience.

EZ Dry Ultra products are available in various sizes, accommodating solvent volumes from 100 mL to 20 L.

December 17th, 2023

emp BIOTECH GmbH Opens Compliance Hotline.
At emp Biotech GmbH, we prioritize transparency, integrity, and accountability.
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We value the well-being of our employees, customers, and stakeholders. When you have a concern about any potential breach of regulation or internal policies within our organization, we encourage you to use our whistleblowing channel.
Click here to access our whistleblowing channel and take an active role in promoting transparency and accountability.

13th to 15th November

emp BIOTECH Delegation Attending Medica 2023.
Are you attending this year’s Medica in Düsseldorf?
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Take this opportunity to meet with delegates from emp Biotech. Three delegates from emp Biotech will be attending Medica between Monday 13th to Wednesday 15th November. Present from emp BIOTECH will be:
• Derek Levison, Managing Director
• Alistair Hurst, Business Development Manager
• Franziska Meier-Hättig, Product Manager
Contact us if you wish to meet with the emp BIOTECH team during Medica.

Optimizing Use of Your Desalting Column

Optimizing Use of
Your Desalting Column

Desalting columns are an essential tool in the field of molecular biology, biochemistry, and protein research. They facilitate the removal of unwanted salts, small molecules, and other contaminants from a sample, purifying and concentrating target molecules in the process.

In this article, we will delve into the importance of optimizing desalting column for effective purification of samples. Desalting columns play a vital role in removing salts and other impurities, ensuring the delivery of high-quality samples for downstream applications.

Optimizing Desalting Column:
Factors to Consider

The following factors should be considered to optimize the
performance of your desalting column:

  1. Sample Preparation: Prior to using the desalting column, ensure that your sample is prepared correctly. This includes selecting the appropriate buffer, pH, and sample concentration. The buffer should be compatible with both the target molecule and the desalting column matrix. Additionally, the pH should be maintained within the optimal range for the target molecule’s stability.
  2. Column Selection: Choose the right desalting column for your specific application. EMP Biotech offers desalting columns covering a wide range of sample volumes. Selecting the appropriate column ensures optimal workflow, purification, and recovery of your target molecule.
  3. Equilibration: Before loading your sample onto the desalting column, it is essential to equilibrate the column with the desired buffer. Proper equilibration removes any preservatives and ensures that the column matrix is in the optimal condition for sample separation. Follow the manufacturer’s guidelines for equilibrating your EMP Biotech desalting column.
  4. Sample Loading: Load your sample onto the desalting column according to the manufacturer’s instructions. Be mindful of the sample volume, as overloading the column can lead to inadequate separation and reduced recovery of the target molecule. For optimal results, ensure that the sample volume does not exceed the recommended loading capacity for your specific desalting column.
  5. Elution: Elute your sample from the desalting column following the manufacturer’s recommended elution protocol. Proper elution techniques ensure the efficient recovery of your target molecule and minimize the presence of contaminants. Be sure to collect the eluted fractions in appropriate collection tubes or vessels to prevent cross-contamination.
  6. Monitoring and Evaluation: Monitor the elution process by measuring the absorbance of the collected fractions at an appropriate wavelength. This will help you track the elution profile of your target molecule and identify the fractions containing the purified molecule. Evaluate the performance of your desalting column by assessing the purity and recovery of the target molecule in the eluted fractions.

Optimizing Desalting Column:
Tips and Best Practices

To further enhance the performance and efficiency of your desalting column, consider the following tips and best practices:

    1. Maintain optimal storage conditions: Store your desalting column according to the manufacturer’s recommendations. This will help preserve the column’s performance and extend its lifespan.
    2. Clean the column, if necessary: If the desalting column’s performance declines, consider cleaning the column following the manufacturer’s guidelines. Proper cleaning can restore the column’s performance and prolong its life.
    3. Monitor the column’s performance over time: Regularly assess the performance of your desalting column by evaluating the purity and recovery of your target molecule. This will help you identify any potential issues and take appropriate action to maintain optimal column performance.

Conclusion

emp BIOTECH desalting columns are valuable tools for the purification and concentration of target molecules in various research applications.

By considering factors such as sample preparation, column selection, equilibration, sample loading, elution, and monitoring, you can optimize the use of your desalting column and achieve consistent, high-quality results. Incorporating tips and best practices such as proper storage, column cleaning, buffer optimization, and gradient elution can further enhance the performance and efficiency of your desalting column, ensuring the success of your biomolecule purification.

Are you purifying proteins or DNA?

Are you purifying proteins or DNA?

Read emp BIOTECH’s latest brochure
on Biomolecule Purification

If you already know emp BIOTECH’s desalting columns, or their SMART Chromatography™ technology, you might not be aware that emp also manufactures a range of resins, available in bulk quantities, that can be applied to a number of purification challenges.

The latest catalogue covers emp’s range in:

  • Affinity chromatography
  • Ion exchange (IEX) purification
  • Hydrophobic interaction chromatography (HIC)
  • Activated resins – for attaching your “favourite” molecules

…and of course, emp BIOTECH’s SEC columns, resins etc. are also covered.

Download:

Biomolecule Purification, Catalog 09/2023